| Category | CELL | L12 | Bacterial Transformation |
| Abstract | The purpose of this experiment was to determine whether it is possible |
| to introduce foreign DNA into a bacteria for which one can transfer |
| other proteins. Bacterial transformation is a process by which bacterial |
| cells take up naked DNA molecules. Of the ways in which bacteria take |
| up DNA from their environment is through their cell wall. Through |
| various heating and cooling procedures, with very explicit guidelines, |
| this project was conducted. E. coli bacteria were used over a three-day |
| period under the supervision of Caitlyn VanGelderen, candidate of a |
| biochemistry and molecular biology at Michigan State University. The |
| plasmid was successfully taken up into the E. coli cells, and the proof is |
| that the colonies grew because if they did not have the plasmid, they |
| would not have been able to grow in the presence of the antibiotics, |
| ampicillin and kanamycin. |
| Bibliography | https://www.ncbi.nlm.nih.gov/books/NBK21993/ |
| https://www.boundless.com/microbiology/textbooks/boundless- |
| microbiology-textbook/microbial-genetics-7/genetic-transfer-in- |
| prokaryotes-81/bacterial-transformation-442-6842/ |