| | Category | CELL | L12 | Bacterial Transformation |
| | Abstract | The purpose of this experiment was to determine whether it is possible |
| | to introduce foreign DNA into a bacteria for which one can transfer |
| | other proteins. Bacterial transformation is a process by which bacterial |
| | cells take up naked DNA molecules. Of the ways in which bacteria take |
| | up DNA from their environment is through their cell wall. Through |
| | various heating and cooling procedures, with very explicit guidelines, |
| | this project was conducted. E. coli bacteria were used over a three-day |
| | period under the supervision of Caitlyn VanGelderen, candidate of a |
| | biochemistry and molecular biology at Michigan State University. The |
| | plasmid was successfully taken up into the E. coli cells, and the proof is |
| | that the colonies grew because if they did not have the plasmid, they |
| | would not have been able to grow in the presence of the antibiotics, |
| | ampicillin and kanamycin. |
| | Bibliography | https://www.ncbi.nlm.nih.gov/books/NBK21993/ |
| | https://www.boundless.com/microbiology/textbooks/boundless- |
| | microbiology-textbook/microbial-genetics-7/genetic-transfer-in- |
| | prokaryotes-81/bacterial-transformation-442-6842/ |